Parallel Bubble Columns with Fed-Batch Technique for Microbial Process Development on a Small Scale

At present, there are a number of commercial small scale shaken systems available on the market with instrumented controllable microbioreactors such as Micro–24 Microreactor System (Pall Corporation, Port Washington, NY) and M2P Biolector, (M2P Labs GmbH, Aachen, Germany). The Micro–24 system is basically an orbital shaken 24–well plate that operates at working volume 3 – 7 mL with 24 independent reactors (deep wells, shaken and sparged) running simultaneously. Each reactor is designed as single use reactor that has the ability to continuously monitor and control the pH, DO and temperature. The reactor aeration is supplied by sparging air from gas feeds that can be controlled individually. Furthermore, pH can be controlled by gas sparging using either dilute ammonia or carbon dioxide directly into the culture medium through a membrane at the bottom of each reactor. Chen et al., (2009) evaluated the Micro–24 system for the mammalian cell culture process development and found the Micro–24 system is suitable as scaledown tool for cell culture application. The result showed that intra-well reproducibility, cell growth, metabolites profiles and protein titres were scalable with 2 L bioreactors.

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Author response for "Holistic process development to mitigate proteolysis of a subunit rotavirus vaccine candidate produced in Pichia pastoris by means of an acid pH pulse during fed‐batch fermentation"

10.1002/btpr.2966/v2/response1 ◽

2020 ◽

Author(s):

M. Lourdes Velez‐Suberbie ◽

Stephen A. Morris ◽

Kawaljit Kaur ◽

John M. Hickey ◽

Sangeeta B. Joshi ◽

...

Keyword(s):

Pichia Pastoris ◽

Batch Fermentation ◽

Process Development ◽

Vaccine Candidate ◽

Author Response ◽

Rotavirus Vaccine ◽

Fed Batch ◽

Holistic Process ◽

Fed Batch Fermentation ◽

A Subunit

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Equalizing growth in high-throughput small scale cultivations via precultures operated in fed-batch mode

Biotechnology and Bioengineering ◽

10.1002/bit.22349 ◽

2009 ◽

Vol 103 (6) ◽

pp. 1095-1102 ◽

Cited By ~ 24

Author(s):

Robert Huber ◽

Marco Scheidle ◽

Barbara Dittrich ◽

Doris Klee ◽

Jochen Büchs

Keyword(s):

High Throughput ◽

Small Scale ◽

Fed Batch ◽

Batch Mode

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Parallel-operated stirred-columns for microbial process development

Biochemical Engineering Journal ◽

10.1016/s1369-703x(02)00010-4 ◽

2002 ◽

Vol 11 (1) ◽

pp. 69-72 ◽

Cited By ~ 16

Author(s):

Dirk Weuster-Botz ◽

Susanne Stevens ◽

Alexander Hawrylenko

Keyword(s):

Process Development ◽

Microbial Process

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Description of a Decentralized Small Scale Digester for Treating Organic Wastes

Environments ◽

10.3390/environments7100078 ◽

2020 ◽

Vol 7 (10) ◽

pp. 78

Author(s):

Rubén González ◽

Daniel Blanco ◽

Judith González-Arias ◽

José García-Cascallana ◽

Xiomar Gómez

Keyword(s):

Organic Loading Rate ◽

Small Scale ◽

Complex Nature ◽

Fed Batch ◽

Batch Mode ◽

Commercial Unit ◽

Start Up ◽

Volatile Solids ◽

Vegetable Wastes ◽

Feeding Substrate

This manuscript deals with the detailed design of a small digestion prototype intended as a commercial unit fully operational to cover the demand for decentralized treatment of wastes. These plants are highly affected by the complex nature of wastes giving rise to different operating problems that should be considered in detail. This paper describes the design and start-up strategy of a small-scale digestion plant with a volume of 8 m3 designed to operate with a hydrolysis pretreatment unit. The plant was designed to treat fruit and vegetable wastes as substrates derived from a local processing food factory. The performance of the plant during fed-batch operation was reported. The strategy of inoculating the reactor only to a third of its original volume and subsequently increasing the volume of the reactor by using the fed-batch mode was inadequate. The acid pH of the feeding substrate resulted in the application of a low organic loading rate with a volumetric variation of just 19.7 L/d. The performance of the plant was evaluated at non-steady state conditions and resulted in excessive destruction of volatile solids due to the low nitrogen content of the feeding substrate. The prototype reported a specific methane production of 232 L/kg volatile solids despite the low feeding rate supplemented.

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Aerogel for Microsystems Thermal Insulation: System Design and Process Development

Heat Transfer: Volume 4 ◽

10.1115/ht2005-72663 ◽

2005 ◽

Cited By ~ 2

Author(s):

Brian Smith ◽

David Romero ◽

Damena Agonafer ◽

Jason Gu ◽

Cristina H. Amon

Keyword(s):

Thermal Insulation ◽

Large Scale ◽

Process Development ◽

System Modeling ◽

Dielectric Constants ◽

Thermal Design ◽

Small Scale ◽

Thermal Testing ◽

High Porosity ◽

Crystal Oscillator

Extreme miniaturization in the microelectronics component market along with the emergence of system-on-chip applications has driven interest in correspondingly small-scale thermal management designs requiring novel material systems. This paper concentrates on aerogel, which is an amorphous, nanoporous dielectric oxide fabricated through a sol-gel process. Its extremely high porosity leads to very low thermal conductivity and dielectric constants. Significant research has been devoted to its electrical properties; however, there are several emerging applications that can leverage the thermal characteristics as well. Two promising applications are investigated in this paper: a monolithically integrated infrared sensor that requires thermal isolation between sensor and silicon substrate, and an ultra-miniature crystal oscillator device which demands thermal insulation of the crystal for low-power operation. This paper identifies the potential benefits of aerogel in these applications through system modeling, demonstrates aerogel’s compatibility with standard low-cost microfabrication techniques, and presents results of thermal testing of aerogel films compared with other microelectronics insulators and available data in the literature. The goal is to explore system thermal design using aerogel while demonstrating its feasibility through experimentation. The combination of numerical simulations, Bayesian surrogate modeling, and process development helps to refine candidate aerogel applications and allow the designer to explore thermal designs which have not previously been possible in large-scale microelectronics system production. This paper was also originally published as part of the Proceedings of the ASME 2005 Pacific Rim Technical Conference and Exhibition on Integration and Packaging of MEMS, NEMS, and Electronic Systems.

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